To comply with Option 2, you’ll need to calculate the weighted impact of the following characteristics of all refrigerants used in your project: 

environmental impact (through destruction of the ozone layer and the concentration of greenhouse gases in the atmosphere);
coolant capacity and refrigerant charge (determined by equipment specs); 
rate of refrigerant leakage (determined by equipment life);
and end-of-life refrigerant loss (largely determined by equipment specs).